Targeted gene suppression through double-stranded RNA application using easy-to-use methods in Arabidopsis thaliana

Abstract RNA interference (RNAi) is an RNA-dependent gene silencing process that regulated by the interaction between RNA-induced complex (RISC) and double-stranded (dsRNA). Exogenous dsRNAs are imported directly into cytoplasm, where they cleaved Dicer short dsRNA fragments of 20–25 base pairs. These fragments, called small interfering RNAs (siRNAs) have sequence-specific with target genes. The guide strand, onto which siRNAs incorporated in RISC interacts mRNA sequence, thereby inducing cleavage degradation messenger (mRNAs) ribonucleases. Recent studies shown plant treatments can induce RNAi. However, application methods delivery systems involved not been well examined. In this study, was introduced to Arabidopsis thaliana two methods: dipping spray. We synthesized designed mRNAs encoding enhanced green fluorescent protein ( EGFP ). After applying , we found obvious reduction GFP expression. This determined using fluorescence microscopy quantitative reverse transcription PCR assess levels auxin-sensitive reporter DR5-EGFP . Our data revealed gene-specific exogenous suppression genes interest whether or spray method used. study therefore provides a foundation for understanding how apply deliver plants.